Strain inhibition of bacterial collagenase is consistent with a collagen fibril uncrimping mechanism in rat tail tendons.

Mechanical strain inhibits bacterial collagenase from cleaving collagen. Additionally, the toe region of a soft tissue's force-elongation curve arises from sequentially engaging collagen fibrils as the tissue lengthens. Together, these phenomena suggest that mechanical strain may gradually inhibit collagenase activity through a soft tissue's toe region. Therefore, this investigation sought to test this hypothesis. 92 rat tail tendon fascicles from 3 female sentinel animals underwent preliminary stiffness tests, and their force-elongation curves were fit to a collagen distribution model. This distribution-based model calculated the force magnitude corresponding to p% of collagen fibril engagement. Specimens were separated into one of five levels of p, and that level of force was maintained for two hours while being exposed to 0.054 U/mL of bacterial collagenase from C. histolyticum. The specimens were strained to failure following the creep test, and the relative reduction in stiffness was quantified to estimate the fraction of digested fibrils. Every 10% additional collagen engagement corresponded to a 6.3% (97% highest density interval: 4.3 - 8.4%) retention of stiffness, which indicated collagenase inhibition. The results of this investigation were consistent with a strain-inhibition hypothesis along with the established uncrimping mechanism in the toe region. These results support an interaction between mechanical strain and collagenolysis, which may be valuable for disease prevention or treatment.

Lower limb postures resembling sitting and standing alter lumbar angles along the passive stiffness curve.

In vivo lumbar passive stiffness is often used to assess time-dependent changes in lumbar tissues and to define the neutral zone. We tested the hypothesis that flexing the hips would alter tension in hip and spine musculature, leading to a more extended passive stiffness curve (i.e., right-shifted), without changes in lumbar stiffness. Twenty participants underwent side-lying passive testing with the lower limbs positioned in Stand, Kneel, and Sit representative postures. Moment-angle curves were constructed from the lumbar angles and the moment at L4/5 and partitioned into three zones. Partially supporting our hypothesis, lumbar stiffness within the low and transition stiffness zones was similar between the Stand and Sit. Contrary to our hypothesis, lumbar angles were significantly larger in the Sit compared to the Stand and Kneel postures at the first and second breakpoints, with average differences of 9.3° or 27.2% of passive range of motion (%PassRoM) in flexion and 5.6° or 16.6 %PassRoM in extension. Increased flexion in the Sit may be linked to increased posterior pelvic tilt and associated lower lumbar vertebrae flexion. Investigators must ensure consistent pelvis and hip positioning when measuring lumbar stiffness. Additionally, the adaptability of the neutral zone to pelvis posture, particularly between standing and sitting, should be considered in ergonomic applications.

Defining the lumbar and trunk-thigh neutral zone from the passive stiffness curve: application to hybrid sit-stand postures and chair design.

Minimal data exist on the neutral position for the lumbar spine, trunk, and thighs when adopting a hybrid posture. This study examined sex differences in the neutral zone lumbar stiffness and the lumbar and trunk-thigh angle boundaries of the neutral zone, and determined if the standing lumbar angle fell within the neutral zone. Passive lumbar flexion and extension moment-angle curves were generated for 31 participants (13 M, 18 F), pooled from two datasets, with trunk-thigh angles available for 10 participants. The neutral zone was defined as the low stiffness zone from both the flexion and extension curves. Males demonstrated significantly greater extensor stiffness. Neutral lumbar and trunk-thigh angles ranged on average -22.2 to 0.2° and 124.2 to 159.6° for males and -17.8 to -1.3° and 143.2 to 159.5° for females, respectively. Standing lumbar angles fell outside the neutral zone for 44% of participants. These neutral zone boundaries may inform kinematics for hybrid chair designs.Practitioner summary: Adoption of a neutral spinal posture may be achieved through hybrid chair design, yet minimal data exists on a physiologically defined neutral zone. Using measures of in vivo lumbar stiffness, the lumbar and trunk-thigh angular boundaries of the neutral zone were defined for both males and females.Abbreviations: EMG: electromyography; MVC: maximal voluntary contraction.

Head supported mass, moment of inertia, neck loads and stability: A simulation study.

Occupations or activities where donning head-supported mass (HSM) is commonplace put operators at an elevated risk of chronic neck pain. Yet, there is no consensus about what features of HSM influence the relative contributions to neck loads. Therefore, we tested four hypotheses that could increase neck loads: (i) HSM increases gravitational moments; (ii) more muscle activation is required to stabilize the head with HSM; (iii) the position of the HSM centre of mass (COM) induces gravitational moments; and (iv) the added moment of inertia (MOI) from HSM increases neck loads during head repositioning tasks. We performed a sensitivity analysis on the C5-C6 compression evaluated from a 24-degree freedom cervical spine model in OpenSim for static and dynamic movement trials. For static trials, we varied the magnitude of HSM, the position of its COM, and developed a novel stability constraint for static optimization. In dynamic trials, we varied HSM and the three principle MOIs. HSM magnitude and compression were linearly related to one another for both static and dynamic trials, with amplification factors varying between 1.9 and 3.9. Similar relationships were found for the COM position, although the relationship between C5-C6 peak compression and MOI in dynamic trials was generally nonlinear. This sensitivity analysis uncovered evidence in favour of hypotheses (i), (ii) and (iii). However, the model's prediction of C5-C6 compression was not overly sensitive to the magnitude of MOI. Therefore, the HSM mass properties may be more influential on neck compression than MOI properties, even during dynamic tasks.

Cyclic loading history alters the joint compression tolerance and regional indentation responses in the cartilaginous endplate.

This study quantified the effect of subthreshold loading histories that differed by joint posture (neutral, flexed), peak loading variation (10%, 20%, 40%), and loading duration (1000, 3000, 5000 cycles) on the post-loading Ultimate Compressive Tolerance (UCT), yield force, and regional Cartilaginous End Plate (CEP) indentation responses (loading stiffness and creep displacement). One hundred and fourteen porcine spinal units were included. Following conditioning and cyclic compression exposures, spinal units were transected and one endplate from each vertebra underwent subsequent UCT or microindentation testing. UCT testing was conducted by compressing a single vertebra at a rate of 3 kN/s using an indenter fabricated to a representative intervertebral disc size and shape. Force and actuator position were sampled at 100 Hz. Non-destructive uniaxial CEP indentation was performed at five surface locations (central, anterior, posterior, right, left) using a Motoman robot and aluminum indenter (3 mm hemisphere). Force and end-effector position were sampled at 10 Hz. A significant three-way interaction was observed for UCT (p = 0.038). Compared to neutral, the UCT was, on average, 1.9 kN less following each flexed loading duration. No effect of variation was observed in flexion; however, 40% variation caused the UCT to decrease by an average of 2.13 kN and 2.06 kN following 3000 and 5000 cycles, respectively. The indentation stiffness in the central CEP mimicked the UCT response. These results demonstrate a profound effect of posture on post-loading UCT and CEP behaviour. Control of peak compression exposures became particularly relevant only when a neutral posture was maintained and beyond the midpoint of the predicated lifespan.

Posture and Helmet Configuration Effects on Joint Reaction Loads in the Middle Cervical Spine.

INTRODUCTION: Between 43 and 97% of helicopter pilots in the Canadian Armed Forces report neck pain. Potential contributing factors include the weight of their helmet, night vision goggles (NVG), and counterweight (CW) combined with deviated neck postures. Therefore, the purpose of this investigation was to quantify changes in neck loads associated with posture, helmet, NVG, and CW.METHODS: Eight male subjects volunteered. They undertook one of five deviated neck postures (flexion, extension, lateral bending, axial rotation) times four configurations (no helmet, helmet only, helmet and NVG, and helmet, NVG, and CW). 3D kinematics and EMG from 10 muscles (5 bilaterally) drove a 3D inverse dynamics, EMG-driven model of the cervical spine which calculated joint compression and shear at C5-C6.RESULTS: The compression in the neutral posture was 116.5 (5.7) N, which increased to 143.7 (11.4) N due to a 12.7 N helmet. NVGs, weighing 7.9 N, also generated this disproportionate increase, where the compression was 164.2 (3.7) N. In flexion or extension, the compression increased with increasing head-supported mass, with a maximum of 315.8 (67.5) N with the CW in flexion. Anteroposterior shear was highest in the lateral bending [34.0 (6.2) N] condition, but was generally low (< 30 N). Mediolateral shear was less than 5 N for all conditions.DISCUSSION: Repositioning the center of gravity of the helmet with either NVGs or CW resulted in posture-specific changes to loading. Posture demonstrated a greater potential to reposition the head segment's center of gravity compared to the helmet design. Therefore, helmet designs which consider repositioning the center of gravity may reduce loads in one posture, but likely exacerbate loading in other postures.Barrett JM, McKinnon CD, Dickerson CR, Laing AC, Callaghan JP. Posture and helmet configuration effects on joint reaction loads in the middle cervical spine. Aerosp Med Hum Perform. 2022; 93(5):458-466.

Cervical Spine Motion Requirements From Night Vision Goggles May Play a Greater Role in Chronic Neck Pain than Helmet Mass Properties.

BACKGROUND: Chronic Neck Pain (CNP) among rotary-wing aircrew is thought to stem from night vision goggles (NVG) and counterweight (CW) systems which displace the centre of mass of the head. This investigation aimed to quantify the loads acting on the neck as a function of movement magnitude (MM), helmet conditions, and movement axes in rapid movements. METHODS: Cervical spine kinematics during rapid head repositioning tasks for flexion-extension (FE) and axial rotation (AR) movements were measured from 15 males and 15 females. Participants moved in either a 35° (Near MM) or 70° arc (Far MM), while donning a helmet, helmet with NVG, helmet with NVG and a typical CW, and a CW Liner (CWL). Measured EMG from three muscles bilaterally and used to drive a biomechanical model to quantify the compression and shear acting at the C5-C6 joint. RESULTS: In AR, the NVGs were associated with the largest compression magnitudes, 252 (24) N. CW conditions decreased the maximum compression to 249 (53) N. For FE, the compression was 340 N for the Far MM trials and 246 N for Near MMs. Changing the helmet configuration only modestly influenced these magnitudes in FE. CONCLUSION: Every 30° of MM increased compression by 57 to 105 N. The reduction of the moment of inertia by 16% in the CWL did not reduce reaction forces. Joint loads scaled proportionately with head-supported weight by a factor of 2.05. The magnitudes of loads suggest a cumulative loading pathway for CNP development.

Are rotational passive stiffness and translational passive stiffness correlated? A porcine in vitro study.

BACKGROUND: Qualitative clinical assessments of spinal stiffness have been demonstrated to show moderate correlations with one-another. We hypothesized that these correlations would improve in an in vitro model of the functional spinal unit. If the stiffness of spinal units are different across loading regimes (e.g. flexion-extension versus shear), then it may provide one explanation as to the variability in findings from clinical assessments, since these tests tend not to discriminate rotational and translational degrees-of-freedom. Therefore, the purpose of this investigation was to quantify the relationships between rotational and translational stiffness measures in vitro. METHODS: Forty-eight porcine cervical spine functional units were used in this investigation (20 C3-C4, 28 C5-C6). While under constant 300 N compressive load, range-of-motion tests for both flexion-extension (± 8 Nm, 0.5 deg./s) and anteroposterior shear (± 400 N, 0.2 mm/s) were conducted, to quantify moment-angle and force-deflection curves. Representative stiffness values were then obtained for flexion, extension, anterior shear, and posterior shear using segmented regression. The correlation matrix between these four measures was then used to explore their potential relationships. FINDINGS: Of the six correlations conducted, only the relationship between posterior shear and extension stiffness was statistically significant (p = 0.014), despite featuring a low correlation coefficient (R2 = 0.123). INTERPRETATION: The poor correlations between stiffness metrics in this study supports the disparate findings of tissue stiffness in vivo. Results from this investigation suggest that clinicians should be cognizant of which degrees-of-freedom they are assessing in the spine, as their stiffness values vary independently.

Characterizing the Mechanical and Viscoelastic Response of the Porcine Facet Joint Capsule Ligament in Response to a Simulated Impact.

The facet capsule ligament (FCL) is a structure in the lumbar spine that constrains motions of the vertebrae. Subfailure loads can produce microdamage resulting in increased laxity, decreased stiffness, and altered viscoelastic responses. Therefore, the purpose of this investigation was to determine the mechanical and viscoelastic properties of the FCL under various magnitudes of strain from control samples and samples that had been through an impact protocol. Two hundred FCL tissue samples were tested (20 control and 180 impacted). Impacted FCL tissue samples were obtained from functional spinal units that had been exposed to one of nine subfailure impact conditions. All specimens underwent the following loading protocol: preconditioning with five cycles of 5% strain, followed by a 30 s rest period, five cycles of 10% strain, and 1 cycle of 10% strain with a hold duration at 10% strain for 240 s (4 min). The same protocol was followed for 30% and 50% strain. Measures of stiffness, hysteresis, and force-relaxation were computed. No significant differences in stiffness were observed for impacted specimens in comparison to control. Impacted specimens from the 8 g flexed and 11 g flexed and neutral conditions exhibited greater hysteresis during the cyclic-30% and cyclic-50% portion of the protocol in comparison to controls. In addition, specimens from the 8 g and 11 g flexed conditions resulted in greater stress decay for the 50%-hold conditions. Results from this study demonstrate viscoelastic changes in FCL samples exposed to moderate and highspeed single impacts in a flexed posture.

The Influence of Simulated Low Speed Vehicle Impacts and Posture on Passive Intervertebral Mechanics.

STUDY DESIGN: An in vitro biomechanics investigation exposing porcine functional spinal units (FSUs) to sudden impact loading although in a flexed, neutral, or extended posture. OBJECTIVE: To investigate the combined effect of impact severity and postural deviation on intervertebral joint mechanics. SUMMARY OF BACKGROUND DATA: To date, no in vitro studies have been conducted to explore lumbar tissue injury potential and altered mechanical properties from exposure to impact forces. Typically, after a motor vehicle collision, the cause of a reported acute onset of low back pain is difficult to identify, with potential soft tissue strain injury sites including the intervertebral disc, facet joint and highly innervated facet joint capsule ligament. METHODS: Seventy-two porcine functional spinal units (36 C34, 36 C56), consisting of 2 adjacent vertebrae, ligaments, and the intervening intervertebral disc were included in the study. Each specimen was randomized to 1 of 3 experimental posture conditions (neutral, flexed, or extended) and assigned to 1 of 3 impact severities representing motor vehicle accident accelerations (4 g, 8 g, and 11 g). Before impact (pre) and after impact (post) flexion-extension and anterior-posterior shear neutral zone testing was completed. RESULTS: A significant two-way interaction was observed between pre-post and impact severity for flexion-extension neutral zone length and stiffness and anterior-posterior shear neutral zone length and stiffness. This was a result of increasedneutral zone range and decreased neutral zone stiffness pre-post for the highest impact severity (11 g), regardless of posture. CONCLUSION: Functional spinal units exposed to the highest severity impact (11 g) had significant neutral zone changes, with increases in joint laxity in flexion-extension and anterior-posterior shear and decreased stiffness, suggesting that soft tissue injury may have occurred. Despite observed main effects of impact severity, no influence of posture was observed.Level of Evidence: N/A.

Strain Response in the Facet Joint Capsule During Physiological Joint Rotation and Translation Following a Simulated Impact Exposure: An In Vitro Porcine Model.

Low back pain (LBP) is frequently reported following rear impact collisions. Knowledge of how the facet joint capsule (FJC) mechanically behaves before and after rear impact collisions may help explain LBP development despite negative radiographic evidence of gross tissue failure. This study quantified the Green strain tensor in the facet joint capsule during rotation and translation range-of-motion tests completed before and following an in vitro simulation of a rear impact collision. Eight FSUs (4 C3-C4, 4 C5-C6) were tested. Following a preload test, FSUs were flexed and extended at 0.5 deg/s until an ±8 N·m moment was achieved. Anterior and posterior joint translation was then applied at 0.2 mm/s until a target ±400 N shear load was imposed. Markers were drawn on the facet capsule surface and their coordinates were tracked during pre- and postimpact range-of-motion tests. Strain was defined as the change in point configuration relative to the determined neutral joint posture. There were no significant differences (p > 0.05) observed in all calculated FJC strain components in rotation and translation before and after the simulated impact. Our results suggest that LBP development resulting from the initiation of strain-induced mechanoreceptors and nociceptors with the facet joint capsule is unlikely following a severe rear impact collision within the boundaries of physiological joint motion.

Passive stiffness changes in the lumbar spine following simulated automotive low speed rear-end collisions.

BACKGROUND: Historically, there has been a lack of focus on the lumbar spine during rear impacts because of the perception that the automotive seat back should protect the lumbar spine from injury. As a result, there have been no studies involving human volunteers to address the risk of low back injury in low velocity rear impact collisions. METHODS: A custom-built crash sled was used to simulate rear impact collisions. Randomized collisions were completed with and without lumbar support. Measures of passive stiffness were obtained prior to impact (Pre), immediately post impact (Post) and 24 h post impact (Post-24). Low back pain reporting was monitored for 24 h following impact exposure. FINDINGS: None of the participants developed clinically significant levels of low back pain after impact. Changes in the passive responses persisted after impact for the length of the low stiffness flexion and extension zone. The length of the low stiffness zone was longer in the Post and Post-24 trial for low stiffness flexion and longer in the Post-24 for low stiffness extension. INTERPRETATION: Findings from this investigation demonstrate that during a laboratory-simulation of an 8 km/h rear-impact collision, young healthy adults did not develop low back pain. Changes in the low stiffness zone of the passive flexion/extension curves were observed following impact and persisted for 24 h. Changes in passive stiffness may lead to changes in the loads and load distributions during movement within the passive structures such as the ligaments and intervertebral discs following impacts.

An Electromyographically Driven Cervical Spine Model in OpenSim.

Relatively few biomechanical models exist aimed at quantifying the mechanical risk factors associated with neck pain. In addition, there is a need to validate spinal-rhythm techniques for inverse dynamics spine models. Therefore, the present investigation was 3-fold: (1) the development of a cervical spine model in OpenSim, (2) a test of a novel spinal-rhythm technique based on minimizing the potential energy in the passive tissues, and (3) comparison of an electromyographically driven approach to estimating compression and shear to other cervical spine models. The authors developed ligament force-deflection and intervertebral joint moment-angle curves from published data. The 218 Hill-type muscle elements, representing 58 muscles, were included and their passive forces validated against in vivo data. Our novel spinal-rhythm technique, based on minimizing the potential energy in the passive tissues, disproportionately assigned motion to the upper cervical spine that was not physiological. Finally, using kinematics and electromyography collected from 8 healthy male volunteers, the authors calculated the compression at C7-T1 as a function of the head-trunk Euler angles. Differences from other models varied from 25.5 to 368.1 N. These differences in forces may result in differences in model geometry, passive components, number of degrees of freedom, or objective functions.

Exploring the influence of impact severity and posture on vertebral joint mechanics in an in-vitro porcine model.

To date, no in vitro studies have been conducted to explore lumbar soft tissue injury potential and altered mechanical properties from exposure to impact forces. After a motor vehicle collision (MVC), the cause of reported acute onset low back pain is difficult to associate with potential soft tissue strain injury sites including the facet joint and innervated facet joint capsule ligament (FJC). Thus, the purpose of this investigation was to quantify intervertebral anterior-posterior (AP) translation and facet joint capsule strain under varying postures and impact severities. Seventy-two porcine spinal units were exposed to three levels of impact severity (4 g, 8 g, 11 g), and posture (Neutral, Flexion, Extension). Impacts were applied using a custom-built impact track that replicated parameters experienced in low to moderate speed rear-end MVCs. Flexion-extension and anterior-posterior shear neutral zone testing were completed prior to impact. AP intervertebral translation and the strain tensor of the facet capsule ligament were measured during impacts. A significant main effect of collision severity was observed for peak AP intervertebral translation (4 g-2.8 ±0.53 mm; 8 g-6.4 ±2.9 mm; 11 g-8.3 ±0.45 mm) and peak FJC shear strain (2.37% strain change from 4 g to 11 g impact severity). Despite observed main effects of impact severity, no influence of posture was observed. This lack of influence of posture and small FJC strain magnitudes suggest that the FJC does not appear to undergo injurious or permanent mechanical changes in response to low-to-moderate MVC impact scenarios.

Model-Aided Design of a Rear-Impact Collision Testing System for In Vivo Investigations.

A collision testing device used to simulate rear-end impacts on human volunteers was developed and validated. The testing device was designed using impact parameters obtained from real crash-test-derived vehicle-to-vehicle rear-end collisions. Experimental results show the proposed testing device achieves repeatable impact parameters well within the reported ranges of real vehicle-to-vehicle rear-end impact simulations reported in the literature. In particular, the device was able to produce a 7.66 (0.30) km/h delta-v collision with a duration of 111.6 (6.2) ms, and a 4.75 (0.29) g peak acceleration.

A novel least-squares method to characterize in-vivo joint functional passive regional stiffness zones.

OBJECTIVE: To present and evaluate a method to objectively quantify the functional regions of joint lumped passive stiffness. BACKGROUND: Joint passive stiffness has an important clinical role in constraining the degrees of freedom at a given joint. Links between passive stiffness and injury, pathology and function may be better understood if joint passive stiffness can be accurately quantified. Thus, a technique was developed to objectively partition passive stiffness curves into 3 linear regions (low, transition and high stiffness). METHODS: The passive stiffness of the lumbar spine is presented as an example. Simulated data was used to determine the sensitivity of the method to Gaussian white noise in force measurements. An experimentally determined lumbar passive flexion curve was used to demonstrate the technique on human data. Breakpoint analysis was employed on the resulting moment-angle cures to partition the curve into low, transition and high stiffness zones. RESULTS: The proposed method was successful at discriminating between the three stiffness zones and quantifying the passive stiffness within each zone. The algorithm had difficulty determining parameters in the low-stiffness zone in the presence of noise. CONCLUSIONS: The proposed method can be used as an objective method to investigate passive stiffness. Breakpoint Analysis can identify the three functional linear zones of passive stiffness. The slopes of these linear regions are then used as a measure of passive stiffness, which have applications in clinical populations and research studies, to assess time varying responses, or changes in stiffness following an intervention.

A one-dimensional collagen-based biomechanical model of passive soft tissue with viscoelasticity and failure.

INTRODUCTION: Strains and sprains of soft tissues, including tendons and ligaments, are frequently occurring injuries. Musculoskeletal models show great promise in prediction and prevention of these injuries. However, these models rarely account for the viscoelastic properties of ligaments and tendons, much less their failure properties. The purpose of this project was to develop, simplify, and analyze a collagen-distribution model to address these limitations. MODEL DEVELOPMENT: A distribution-moment approximation was applied to an existing partial differential equation model to reduce its computational complexity. The resulting model was equipped with a Voigt model in series, which endowed it with viscoelastic properties in addition to failure properties. RESULTS: The model was able to reproduce the characteristic toe, linear, and failure regions ubiquitous throughout in-vitro tests on tissue specimens. In addition, it was able to reproduce a tri-phasic creep test consisting of an initial deformation, a steady-state, and failure. Stress-relaxation and hysteresis were also reproducible by the model. DISCUSSION AND CONCLUSION: The ability to reproduce so many characteristics of biological tissues suggests more bio-fidelity was achieved by the reduced model was other currently available models. Future work to further improve its bio-fidelity is proposed for specific tendons and ligaments.

The rate of tendon failure in a collagen fibre recruitment-based model.

Accurate characterization of the mechanical response of collagenous tissues is critical for investigations into mechanisms of soft tissue injury. These tissues are inherently viscoelastic, exhibiting strain-rate dependent stiffnesses, creep, and stress-relaxation. The strain-rate features of the failure portion of the stress-strain curve are less well developed. Collagen-distribution based models are improving and capable of reproducing the non-linear aspects of the elastic response of soft tissues, but still require parameterization of failure regions. Therefore, the purpose of this investigation, was to determine whether the parameters characterizing the rate of damage accumulation in a collagen-distribution model are proportional to strain rate. Fifty rat tail tendons were subjected to one of five different strain rates (0.01, 0.05, 0.1, 0.15, 0.20 s-1) until failure in an uni-axial strain test. To test the hypothesis that the parameters associated with damage rate are proportional to strain rate, a collagen distribution model was employed with the parameters describing the rate of fibre damage being obtained by least-squares and regressed against the strain rate. The breaking function was found to be proportional to strain rate, with a proportionality constant of 60.7 s-1. Properties characterizing the failure portion of the stress-strain curves for rat tail tendons are also reported. The Young's Modulus did not vary with strain rate and was found to be 103.3 ± 49.5 MPa. Similarly, failure stresses and strains did not vary across the strain rates tested, and were 15.6 ± 6.1 MPa and 32.2 ± 9.1%, respectively.

A Comparison of Clinical Spinal Mobility Measures to Experimentally Derived Lumbar Spine Passive Stiffness.

Spinal stiffness and mobility assessments vary between clinical and research settings, potentially hindering the understanding and treatment of low back pain. A total of 71 healthy participants were evaluated using 2 clinical assessments (posteroanterior spring and passive intervertebral motion) and 2 quantitative measures: lumped mechanical stiffness of the lumbar spine and local tissue stiffness (lumbar erector spinae and supraspinous ligament) measured via myotonometry. The authors hypothesized that clinical, mechanical, and local tissue measures would be correlated, that clinical tests would not alter mechanical stiffness, and that males would demonstrate greater lumbar stiffness than females. Clinical, lumped mechanical, and tissue stiffness were not correlated; however, gradings from the posteroanterior spring and passive intervertebral motion tests were positively correlated with each other. Clinical assessments had no effect on lumped mechanical stiffness. The males had greater lumped mechanical and lumbar erector spinae stiffness compared with the females. The lack of correlation between clinical, tissue, and lumped mechanical measures of spinal stiffness indicates that the use of the term "stiffness" by clinicians may require reevaluation; clinicians should be confident that they are not altering mechanical stiffness of the spine through segmental mobility assessments; and greater resting lumbar erector stiffness in males suggests that sex should be considered in the assessment and treatment of the low back.

A Mobile Application to Measure Trunk Flexion Angles in Lifting Tasks.

Occupational Abstract The aim of this project was to develop and test an application capable of quickly and repeatedly measuring trunk flexion angles during sagittal plane lifting tasks. The developed application uses the built-in accelerometer in mobile devices to approximate trunk flexion angle, as the user follows an operator as they perform a lift. A black line is superimposed over the camera feed, allowing the user to approximate the angle of inclination of a line connecting the operator's seventh cervical and first sacral vertebrae-thereby estimating the trunk flexion angle. The magnitude of this angle and its velocity have been linked to the development of occupational low back pain; thus the application provides ergonomists a more refined means of screening tasks beyond currently available survey tools.

TECHNICAL ABSTRACT Background The majority of quantitative postural analysis tools used in biomechanics laboratories are either infeasible or impractical for applied ergonomic field use. Survey tools do exist but are subjective in nature. Purpose To develop an application for handheld mobile devices that can quickly, reliably, and accurately measure the trunk flexion angle in order to afford more detailed and objective ergonomic analyses. Methods The application, Trunk Angle Goniometer (TAG), was programed using Xcode (Apple Inc. Cupertino, CA). Sixteen participants measured the trunk angle of lifts in the sagittal plane using TAG installed on an iPad (Apple Inc., Cupertino, CA). To establish the accuracy of the application, comparisons were made to gold standard (manual anatomical landmark digitization) measures of maximum trunk angle, maximum trunk velocity, and the root-mean square (RMS) difference between trunk angle time histories. Precision was also assessed between raters (inter-rater reliability), between trials assessing the same lift (intra-rater reliability) and between trials assessing similar lifts (test-retest reliability). Results TAG generally underestimated the true magnitude of trunk flexion by 5 ° to 15 °, and overestimated flexion velocity by approximately 10 °/sec. RMS errors were between 8.6 ° and 13.4 °. Performance measures showed fair to good test-retest reliability between 0.631 and 0.709. Overall the application had an excellent inter-rater reliability above 0.95 for all measures; however, suffered from low intra-rater reliability (0.381 to 0.520) but these dramatically increased when averages were taken across multiple trials (from 0.739 to 0.838). Conclusions TAG performed well for quantifying angles in the sagittal plane. The approach has the added benefit of being able to assess lifting tasks in real time, combined with its relatively cheap cost, the approach shows promise for field-work and assessments.